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第一篇论文摘要：STAT3-siRNA对人结直肠癌细胞SW480的干涉作用(英文)
【目的】应用经小干扰RNA(short interfere RNA,siRNA)表达盒介导的RNAi对结直肠癌细胞中的STAT3基因进行干涉作用以研究STAT3-siRNA对STAT3基因表达阳性的结直肠癌细胞凋亡的影响.【方法】用脂质体转染试剂将STAT3-siRNA表达盒(STAT3-siRNA expression cassettes,STAT3-SECs)体外转染至人结直肠癌SW480细胞及人成纤维细胞中.于48 h后收集细胞,先经荧光染色方法观察细胞表象变化,再通过流式细胞仪检测人结直肠癌SW480细胞凋亡情况,最后分别提取细胞总RNA,用RT-PCR测定STAT3基因在mRNA水平的表达.【结果】SW480 STAT3-SECs组的细胞可见凋亡小体,出现明显的凋亡现象,而其它实验组均未出现明显的凋亡现象.流式细胞术结果显示SW480 STAT3-SECs组中凋亡细胞百分比由0.2%增加至26.0%,S期细胞比率由32.3%下降至9.2%.在mRNA水平上,SW480 STAT3-SECs组细胞的STAT3基因表达减少了(62.16±,12.50)%,显著低于SW480对照组(P<,0.01).【结论】应用RNAi技术沉默STAT3基因可以降低人结直肠癌SW480细胞中STAT3的表达,诱导细胞的凋亡.
第二篇摘要范文：降低Pin1可以抑制大肠癌细胞SW620的侵袭转移能力(英文)
Objective:The aim of our study was to investigate the effect of Pin1 on the expression of MMP-2 and MMP-9 in human colorectal carcinoma SW620 cells. Methods: We constructed a eukaryotic expression vector of RNA interfering (shRNA) for Pin1 gene (pGenesil-1-Pin1), and then observed its expression in SW620 cells by Western blotting. The cells motility were tested by wound healing assay and Boyden chamber assay. The protein levels and activity of MMP-2 and MMP-9 were tested by Western blotting and Gelatin zymography in SW620 cells after transfected with pGenesil-1-PIN1. Results: pGenesil-1-PIN1 was successfully constructed, which was confirmed by sequencing. Silencing the Pin1 by RNAi significantly decreased the cells motility from 96.4±,3.9 per field (×,10 objective) to 52.7±,4.4 per field (P<,0.05, Student',s t-test) for SW620 cells transfected with pGenesil-1-PIN1 (SW620/p-shRNA) in Boyden chamber assay, and reduced the MMP-2 and MMP-9 expressions and activity in SW620 cells. The protein relative levels of MMP-2 were 0.32±,0.04 for SW620/p-shRNA, and 0.76±,0.03 for SW620/p-Con, MMP-9 were 0.41±,0.09 for SW620/p-shRNA, and 0.94±,0.07 for SW620/p-Con (p<,0.05). Conclusion: Inhibited Pin1 expression may contribute to the suppression of the invasive and metastatic capacity of colon cancer cells in vitro.
| 有关论文范文主题研究: | 关于sw英文论文例文 | 大学生适用: | 3000字在职论文、2000字学位论文 |
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| 所属大学生专业类别: | sw英文学科 | 论文题目推荐度: | 优质sw英文论文摘要范文选题 |
第三篇sw英文论文摘要：矮杆大穗高产小麦育种亲本SW3243重要农艺性状特性及育种应用效果(英文)
[目的]研究小麦亲本材料SW3243(组合为1426/4/IR68-77/YAA//ALD‘S’/3/YAZ//ST2022/983)的育种利用价值.[方法]对SW3243的选育、农艺特性、部分品质相关遗传性状、1BL/1RS易位系及育种的应用情况进行了分析.[结果]SW3243具有如下特点:SW3243高抗条中29、30,对抗性基因不具有抑制作用,具有矮杆、多花、大穗、高产、迟播早熟等显著特点,SW3243的高分子量谷蛋白亚基(HMW-GS)组成为1,7+8,2+12,含有232bp片段的PPO等位基因,Waxy蛋白亚基没有缺失,籽粒含有少量迟熟α-淀粉酶,SW3243含1BL/1RS易位系,但与供试易位系具有明显不同,利用SW3243的优良农艺特性育成了一批高产新品系,其中有42个衍生系参加了国家和四川省区试,育成国家、省级审定小麦新品种10个(次),SW3243及其衍生品种已累计推广433万hm2以上.[结论]麦材料SW3243具有优良的农艺性状,是四川小麦育种的重要亲本,研究结果将为小麦种质资源创新和高产育种提供信息参考.
第四篇sw英文论文摘要模板：转染AP-2α基因对结肠癌SW620细胞增殖及凋亡的影响(英文)
Objective:We investigated the effects of exogenous AP-2α gene on SW620 cell cycle, apoptosis and proliferation. Methods:The Plasmid pcDNA3.1(+)-AP-2α was transfected into colorectal carcinoma SW620 cells line by liposome mediation for transient expression. After AP-2α transfected SW620 cells, the exogenous AP-2α mRNA and protein express were determined by the method of Real-time PCR and Western blot. In order to elucidate the effect of expression of exogenous AP-2α gene on the colorectal cancer cell SW620, the proliferation rates were analyzed by growth curves for cells including SW620, pcDNA3.1(+)/SW620 and pcDNA3.1(+)-AP-2α/SW620. At same time, the apoptotic rate of cells and the cell cycle analysis were also tested by flow cytometry. Results: The mRNA and protein expressions of AP-2α gene could be enhanced by transfecting of pcDNA3.1(+)-AP-2α recombinant plasmid into SW620 cell. The cell growth rates of SW620 cells transfected with pcDNA3.1(+)/AP-2α were slower than those transfected with pcDNA3.1(+) or untransfected. The apoptotic rates were increased compared with pcDNA3.1(+)/SW620 and SW620 (P<,0.05), which indicated that over-expression of AP-2α gene could efficiently inhibit the growth of SW620 cells and induce apoptosis. FCM analyses indicated that the cells being arrested in G1 phase. Conclusion: AP-2α gene can be efficiently transfected into SW620 cells and over-expression AP-2α protein in the transfected cells. AP-2α induces G1 arrest and apoptosis, suppressive effect on cell growth.
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第五篇sw英文论文摘要怎么写：ERK通路可通过调节mdr-1和RRM1基因的表达参与诱导胰腺癌细胞株SW1990的吉西他滨化疗耐药(英文)
Objective: To investigate the relationship between extracellular signal-regulated kinase (ERK) pathway, multidrug resistance gene (mdr-1), ribonucleotide reductase M1 (RRM1) gene and their roles in gemcitabine (GEM) chemoresistance in pancreatic cancer cell line SW1990. Methods: The GEM-resistance cell model was constructed by a stepwise method. Immunohistochemistry was used to measure the expression of ERK protein (ERK1/2) in the established cell strains in a semiquantitative way. The mRNA expression of mdr-1 and RRM1 were detected by RT-PCR. MTT assay was performed to determine the IC50 value. Results: The established GEM-resistant cell strains were able to gain stable growth and passage ability in the medium contained different concentration levels of GEM (0, 30, 60, 100, 150 and 200 nmol/L). The expression of ERK protein, mdr-1 and RRM1 gene were elevated accompanied by the increase of GEM concentration. There was a highly positive correlation between mdr-1, RRM1 expression and GEM-resistance level (r 等于 0.960, P 等于 0.002 and r 等于 0.966, P 等于 0.002). The expression of ERK protein also correlated with the mdr-1 and RRM1 level (r 等于 -0.943, P 等于 0.005 and r 等于 -0.883, P 等于 0.02). At the GEM-resistance level of 200 nmol/L, the grey scale value of ERK1/2 was 164.22 ±, 13.17, mdr-1/β-actin and RRM1/β-actin were 1.41 ±, 0.04 and 1.45 ±, 0.18, respectively, after treated with ERK pathway inhibitor U0126, these values synchronously decreased to 186.85 ±, 13.14, 0.2 3±, 0.02 and 0.21 ±, 0.03, respectively, inversely, the ERK1/2 grey scale value was 106.55 ±, 16.45, mdr-1/β-actin and RRM1/β-actin were 1.50 ±, 0.07 and 1.52 ±, 0.12, respectively, which presented a tendency of synchronously increase after treated with ERK pathway activator EGF. The IC50 values in GEM-resistant cells of 0 nmol/L and 200 nmol/L levels were 4.104 and 10.20, respectively. After treated with U0126, these values decreased to 3.26 and 4.50, respectively, while treated with EGF, the IC50 values increased to 8.89 and 17.17, respectively. Conclusion: The ERK pathway may induce the GEM-chemoresistance in pancreatic cell line SW1990 through the participation in the regulation of the mdr-1 and RRM1 gene expression.
第六篇摘要范文：降低Pin1对大肠癌SW620细胞的增殖和凋亡能力的影响(英文)
Objective:The aim of our study was to investigate the effects of Pin1 reduction on SW620 cell proliferation and apoptosis in human colorectal carcinoma.Methods:We constructed a plasmid of RNA interfering(shRNA) for Pin1 gene(pGenesl-1-Pin1),then the plasmid was transfected into colorectal carcinoma SW620 cells line by liposome mediation.The protein expression of Pin1 was tested by Western blotting.The proliferation rate was analyzed by MTT and the apoptotic rate of cells was tested by flow cytometry.In order to explain further the effect of Pin1 in SW620 cells,the protein level of Bcl-2 was analyzed by Western blotting.Results:pGenesil-1-Pin1 plasmid was successfully constructed and confirmed by sequencing.The protein relative levels of Pin1 were 0.06 ±, 0.04 for the P-shRNA/SW620 cells,and 0.32 ±, 0.09 for the P-Con/SW620 cells.The cell growth rate of SW620 cells was slower while the apoptotic rate was increased after transfection with pGenesil-Pin1 plasmid,and the apoptotic rate was 12.38% ±, 1.55% for the P-shRNA/SW620 group.At the same time,we found that the protein expression of Bcl-2 was also reduced.The results were 0.13 ±, 0.04 for the P-shRNA/SW620 cells,and 0.36 ±, 0.08 for the P-Con/SW620 cells.Conclusion:Inhibited Pin1 expression may suppress the cell proliferation and promote apoptosis of colorectal carcinoma cells in vitro.
第七篇sw英文论文摘要范文：沉默Sp1抑制大肠癌细胞SW480的端粒酶活性并促进细胞凋亡(英文)
Objective:The aim of the study was to examine the effect of Sp1 on the expression of the human telomerase reverse transcriptase(hTERT) gene in human colorectal carcinoma SW480 cells.Methods:The Sp1 shRNA plasmid was transfected into colorectal carcinoma SW480 cells line by liposome mediation for transient expression.After Sp1 shRNA plasmid transfected SW480 cells,the exogenous Sp1 protein expression was determined by the method of Western blot.At same time,hTERT mRNA expression was detected by RT-PCR,telomerase activity was determined by the telomeric repeat amplification protocol(TRAP) assay,and the apoptotic rate of cells was also tested by flow cytometry.Results:The protein expressions of Sp1 gene could be reduce by transfecting of pGenesil-1-Sp1(+) recombinant plasmid into SW480 cells.The apoptotic rate was increased compared with pGenesil-1-Sp1(-)/SW480 and SW480(P <, 0.05),which indicated that lowexpression of Sp1 gene could lead to low level of telomerase activity and induce apoptosis.Conclusion:Silencing Sp1 may suppress the activity of telomerase by inhabiting hTERT gene expression.
第八篇sw英文论文摘要格式：新型铂(II)类配合物对大肠癌SW620细胞株的体外抑瘤作用及药物作用靶点研究(英文)
Objective:The aim of our study was to evaluate the in vitro antitumor activity of two novel platinum-based(II) complexes(2.3-pyridinedicarboxylic acid dehydrate platinum and 2.3-pyrazinedicarboxylic acid dehydrate platinum),which were concurrently provided with hydrophilic carboxyl group and lipophilic pyrazinyl or pyridyl group,on SW620 colorectal cancer cell line and the impact of the two compounds on the cell cycle and apoptosis of the cells when compared with the oxaliplatin,desiring the new ligand combined with hydrophilic and lipophilic properties would facilitate the transportation and transmembrane of the drugs,showing a better antitumor activity.Methods:After SW620 cells were treated with different doses of the three platinum-based agents for 24,48 and 72 h,the cell proliferation inhibition rate was determined using methyl thiazolyl tetrazolium(MTT) assay,the morphology of cells were evaluated under inverted microscope,the changes in cell cycle were determined using flow cytometry,the percent apoptosis was measured using Annexin V/PI double staining and the micromorphology of the cells after drug exposure was evaluated using scanning electron microscopy.Results:The evaluation on the proliferation inhibition rate revealed that the three platinum-based agents inhibited the SW620 cells in a time-and dose-dependent manner and showed different strengths as pyridine >, pyrazine >, Oxa.Under optical microscope,the morphological changes such as cell shrinkage,round cells and dead cells were frequently observed after drug exposure.Cell cycle determination showed that all of the three agents could function to block the cells converting from phase S to phase G2M.Apoptosis evaluation revealed that the three agents promoted the apoptosis of SW620 cells in a time-and dose-dependent manner and showed different strengths as pyridine >, pyrazine >, Oxa.Typical early and late apoptotic morphological changes could be detected during electron microscopy.Conclusion:The two novel platinum-based(II) complexes showed a stronger antitumor effect on SW620 cells than oxaliplatin,with the targeted site at a certain phase of cell cycle and apoptosis.
第九篇sw英文论文摘要：结肠癌细胞系SW480对不同化疗药物化疗敏感性的研究(英文)
Objective:The aim of this study was to investigate the sensitivity of chemotherapeutic agents 5-FU,cisplatin(DDP) or TAXOL on colon cancer cell line SW480 with different methods,to find out the best examine time period for further study of chemotherapeutic sensitivities.Methods:The SW480 cell was treated with 5-FU(200μg/mL),DDP(150μg/mL) or TAXOL(50μg/mL) respectively for 4h,8h or 12h.MTT assay was used to examine the cell survival rate,Annexin V/PI assay was used to analysis the apoptosis rate,Western Blot assay was applied to examine the expression of apoptotic protein.Results:(1) Results of MTT assay showed that the survival rate of SW480 cells at 4h,8h or 12h was:5-FU(200μg/mL)96.0%±,8.2%,85.4%±,7.8%,74.4% ±,10.2%(P<,0.05),DDP(150μg/mL) 99.0%±,6.4%,88.7%±,4.7%(P<,0.05),46.9%±,2.6%(P<,0.01),TAXOL(50μg/mL) 51.5%±,4.2%(P<,0.01),31.9%±,3.1%,17.6%±,2.3%,or blank group 97.2%±,5.8%,98.7%±,7.2%,97.5%±,7.5% respectively.(2) The apoptosis rate of cancer cells at 4h,8h or 12h was:control group:3.4%±,0.2%,6.2%±,0.4%,7.0%±,0.5%,5-FU(200μg/mL) 4.0%±,0.3%,4.8%±,0.4%,7.7%±,0.7%,DDP(150μg/mL) 8.5%±,0.9%,18.6%±,1.6%(P<,0.05),67.0%±,6.2%(P<,0.01),or TAXOL(50μg/mL) 32.0%±,5.2%(P<,0.01),76.6%±,8.5%,94.0% ±,8.2%,respectively.(3) Western Blot assay showed that the expression of apoptosis associated protein.PARP,X-linked inhibitor of apoptasis(XIAP),Caspase-9 and Bcl-xL were changed.Conclusion:The sensitivity of chemotherapy could be assessed by MTT assay,Annexin V/PI assay and Western Blot.The best examine time of the three chemotherapuc agents was 5-FU(200μg/mL):>,12h,DDP(150μg/mL):8-12h,or TAXOL(50μg/mL):<,4h.
第十篇摘要范文：构建人突变型p27基因重组腺病毒在结肠癌细胞SW480中的表达(英文)
背景:大肠癌的发病率呈明显上升趋势.以往的治疗主要为手术治疗、化学治疗和放射治疗.目前基因疗法已应用于大肠癌治疗的临床实践中.目的:构建复制缺陷型重组hp27mt基因腺病毒并检测其在SW480细胞中的表达,探讨腺病毒载体用于基因治疗的可行性及突变p27的抗肿瘤特性.设计:非随机对照实验.单位:郧阳医学院附属人民医院消化内科,郧阳医学院临床医学研究所,武汉大学中南医院消化内科.材料:实验于2002-01/2003-09在郧阳医学院临床医学研究所完成.AgeⅠ,NheⅠ,KpnⅠ,PacⅠ和PmeⅠ等限制性内切酶,Tag酶,T4DNA连接酶,Westernblot检测试剂盒,鼠抗人p27kip1多抗,辣根过氧化物酶标记的羊抗鼠IgG单抗,pORF9-hp27mt质粒,腺病毒骨架质粒pAdeasy-1,穿梭质粒pShuttle-CMV,LacZ重组腺病毒,脂质体,结肠癌细胞株SW480.方法:①hp27mt自载体pORF9-hp27mt中切出,经2次亚克隆,插入到穿梭质粒pShuttle-CMV中,形成转移质粒pShuttle-CMV-hp27mt,然后再用PmeI线性化的pShuttle-CMV-hp27mt转化含腺病毒骨架质粒pAdeasy-1的超感受态BJ5183,使其在细菌内发生同源重组.重组质粒鉴定正确后经PacI酶切,转入Ad293细胞,包装成重组腺病毒Ad-hp27mt.采用聚合酶链反应方法对重组腺病毒进行鉴定,用紫外分光光度计进行滴度测定.②用重组p27mt的腺病毒Ad-p27mt感染大肠癌细胞SW480,应用WesternBlot检测p27蛋白的表达.主要观察指标:①腺病毒在Ad293细胞中的包装过程.②Ad-p27mt的聚合酶链反应检测Ad-p27mt转染大肠癌细胞后的p27mt的表达.结果:①用含pShuttle-CMV-hp27mt转化含pAdeasy-1的超感受态BJ5183后,可获得约30%的阳性重组质粒.②聚合酶链反应检测表明重组腺病毒DNA中含有目的基因.重组腺病毒滴度为7.95×,1015pfu/L.③转染大肠癌细胞后,p27在SW480中获得了高表达,而未转染细胞和LacZ重组腺病毒转染的细胞中仅有低表达.结论:复制缺陷重组腺病毒能有效介导突变p27基因在肿瘤细胞内表达,为观察突变p27基因对大肠癌的治疗作用提供了有效的基因转移载体.
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